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hygromycin concentration for e-coli selection


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#1 m_rajaee

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Posted 23 August 2011 - 04:43 AM

hi
thanks to all for helping me doing the cotransformation,
now I have the 3 plasmid in my ecoli and I want to do the hygromycin selection,

dose anybody know how much is the concentration of the hygromycin for ecoli selection?

regards

#2 prodes

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Posted 23 August 2011 - 09:19 AM

Hygromycin B is normally used at a concentration of 50-200 µg/ml in mammalian cells and 100 µg/ml in bacteria.




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