Posted 23 August 2011 - 02:51 AM
I'm working on probiotic project. Currently I'm isolating bacteria from dairy product. Recently, I sampled a commercial yogurt product which claimed to have acidophilus, thermophilus and bifidobacteria. Using MRS broth and plated to MRS agar, the only species obtained were probbably yeast and cocci. a few plates do have lactobacillus but mixed with yeast. I have sub-cultured the bacteria 4x so far by re-streaking for single colony on MRS agar but still, I only obtained yeast mixed with cocci and bacilli. Please help me, if anyone knows on any specific media to isolate bifido and lactobacillus. I attached herewith the image of my gram staining for reference.
Posted 23 August 2011 - 05:30 AM
http://www.sciencedi...167701297000250 (it can also be used for yoghurt)
There are some researchpapers out there that speak about specific media for bigidobacteria or lactobacillus.
Take for example this paper: http://www.ncbi.nlm....icles/PMC91699/
its specifically speaking about the isolation of Bifidobacterium spp. from yoghurt...
I dont see why you only used MRS till now.
Edited by pito, 23 August 2011 - 05:38 AM.
If you don't know it, then ask it! Better to ask and look foolish to some than not ask and stay stupid.