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Lactobacillus Isolation and Identification

lactobacillus gram staining goats milk

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#1 ezniidrus

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Posted 23 August 2011 - 02:51 AM

Hi forum members,

I'm working on probiotic project. Currently I'm isolating bacteria from dairy product. Recently, I sampled a commercial yogurt product which claimed to have acidophilus, thermophilus and bifidobacteria. Using MRS broth and plated to MRS agar, the only species obtained were probbably yeast and cocci. a few plates do have lactobacillus but mixed with yeast. I have sub-cultured the bacteria 4x so far by re-streaking for single colony on MRS agar but still, I only obtained yeast mixed with cocci and bacilli. Please help me, if anyone knows on any specific media to isolate bifido and lactobacillus. I attached herewith the image of my gram staining for reference.ABT 1st gram stain mix cult.jpg

#2 pito

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Posted 23 August 2011 - 05:30 AM

Check

http://www.anaerobes...-Selective-Agar
http://www.bd.com/eu...A/PA-254546.pdf

http://www.sciencedi...167701297000250 (it can also be used for yoghurt)
http://www.ncbi.nlm..../pubmed/9921834

There are some researchpapers out there that speak about specific media for bigidobacteria or lactobacillus.

Take for example this paper: http://www.ncbi.nlm....icles/PMC91699/
its specifically speaking about the isolation of Bifidobacterium spp. from yoghurt...
I dont see why you only used MRS till now.

Edited by pito, 23 August 2011 - 05:38 AM.

If you don't know it, then ask it! Better to ask and look foolish to some than not ask and stay stupid.






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