Problem in Stable transfectionBlasticidin
Posted 22 August 2011 - 07:23 PM
Posted 22 August 2011 - 08:51 PM
Posted 23 August 2011 - 08:04 PM
yes, last time i do the optimisation in 2, 4, 6,8,and 10ug/ml and in 6 ug/ml and above,all cell was die. so in frist time, i select in 6ug/ml. For 2nd time transfection I increase to 10 ug/ml for selection, after14 days, I subculture to 2 flask, one is 10ug/ml other is 50ug/ml, but the cell is still survive. Can I try to other cell line like HEK293, may be the CHO cell is gaint the resistance gene?or the pBlast is not in CHO cell?
Posted 14 May 2012 - 12:13 PM