RNA isolation from frozen cells
Posted 22 August 2011 - 09:06 AM
Posted 22 August 2011 - 02:23 PM
Next time: after treating your cells, rinse well with PBS, aspirate, add 1 mL of Trizol (for a T25 flask), scrape dissolved cells up and put in a tube, then freeze. The Trizol will protect the RNA from degradation, and can be stored at -80 for years with no apparent ill effects.
Posted 24 August 2011 - 02:29 AM
I never trust anything that can't be doubted.