I'm trying to set-up BrDU IHC in lesioned brain and I found that it is surprisingly not working. Everything goes perfect for the age-matched controls, that had the same perfusion, storage, cutting and IHC. But on my lesioned samples I see no +cells at all, which is quite weird as I already characterized them by PCNA, ki67, phh3, etc.
Has anyone find any similar problem? I'm trying not to change things in the protocol, as different cycles with boiling citrate or different T with HCl...
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BrDU in lesioned brain
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