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Thrombin removel His tag protein digestion


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#1 Srinivas Penumutchu

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Posted 21 August 2011 - 07:30 AM

After thrombin cutting of his - tagged protein , Iam unable to purify the protein from by using size exclusion chromatography .always showing the contamination of thrombin residuals as per sds page .i am using PBS buffer for throbin cutting .
could you please give any suggestion to remove throbin removal ?

#2 mdfenko

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Posted 22 August 2011 - 12:39 PM

run the digest through a nickel column. the his tag and undigested protein should bind and the digested protein should pass through.
talent does what it can
genius does what it must
i do what i get paid to do




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