how to determine a plant promoter activity in bacteria
Posted 20 August 2011 - 06:06 AM
i am studying on a plant gene promoter. i identify its sequence but i want to determine its activity. I can' t do it by plant cell culture in my lab so i must try it by bacteria. Does anybody know or offer a protochol or a kit for this?I want to clone this promoter and determine its activity in bacteria by calculating luminometer. I am waiting for your advices.
Posted 20 August 2011 - 06:19 AM
Posted 21 August 2011 - 04:47 AM
Posted 21 August 2011 - 06:17 AM
Posted 21 August 2011 - 07:02 AM
best regards to everyone...
Posted 04 September 2011 - 11:37 PM
Posted 05 September 2011 - 06:54 AM
Posted 20 November 2011 - 12:29 AM
did anyone try clontech pmetluc reporter for plant promoter activity test in yeast? or do you know if it is posssible to do?
Posted 24 November 2011 - 10:27 PM
Posted 29 November 2011 - 05:27 AM
Posted 29 November 2011 - 05:44 AM
Hmm first maybe you should find the system for yeast and do transfection once you got one...but based on what i know, normally they already contained promoter in the plasmid...otherwise you need to get the backbone that suit for yeast transformation and construct all by yourself...it will takes time, try to search transformation in yeast let see what you will get...I guess transformation in yeast is not rare, so you will have lot of method just in different strategy..Do you really need to use that promoter? i mean if you cant try it directly on plant it's hard to know the effectiveness of the pomoter right? maybe if you can do transgenic plant, agrobacterium can help you but for me yeast can work too...as long as you can get suitable system for (plasmid) once you insert Promoter+Gene then just use electroporation ... hope it works..