Periplasmic Expression - Your Experience...
Posted 19 August 2011 - 05:37 PM
I wish to express my protein so that it will be secreted into the periplasm. One issue is, no one in the lab actually attempted it and there are many info available but not a specific protocol (that I saw) with bacterial strains, expression vectors, helper stuff (e.g. pTUM4). I would also like to hear about your experience with periplasmic expression and what do you use and what you saw.
Posted 22 August 2011 - 12:05 AM
Posted 22 August 2011 - 12:59 PM