DNA degradation (from human blood)
Posted 19 August 2011 - 06:07 AM
I use the same method for their (same person)buccal cells and saliva, the DNA were intact, a nice band was observed for all on gel.
Why can't i get a nice band for DNA from human blood like from buccal/saliva?
Anyone has any idea?
Posted 21 August 2011 - 03:59 AM
What do you store your DNA in? On what temperature?
You said you observed a nice band, you mean putting just DNA on gel or PCR reaction? Did you check your DNA on gel just after the isolation to see if it didn't degraded in the process?
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