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Fc fusion protein smearing on SDS PAGE

fc fusion smear sds page

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#1 z1e2b3r4

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Posted 18 August 2011 - 11:19 AM

I have several Fc fusion proteins that when I run non-reduced on sds-page (nupage gel) I see a smear from where the protein band would be if it was reduced up to the non reduced molecular weight. Do you know what would be causing this? The sample is not in any reducing conditions. Thanks!smear gel.JPG

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#2 mdfenko

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Posted 18 August 2011 - 11:24 AM

you may be experiencing some dissociation caused by the presence of sds.
talent does what it can
genius does what it must
i do what i get paid to do

#3 proteaMatt

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Posted 19 August 2011 - 06:11 AM

If you are using the invitrogen precast gels they recommend using their Tris-glycine native running buffer. The recipe for it would be the same as your standard TGS, just omit the SDS.
Lab Technician at Protea Biosciences




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