6 replies to this topic

[pathlily]

Hello!

The viscosity of Triton X-100 is pretty annoying to deal with on a day-today basis so I was thinking of making myself a 10% or 20% stock solution.   Since the density of triton is known, and since (I find it) so difficult to pipette it accurately, would it be more sensible to do this by weight?

Do you have any tips on making a Triton X-100 stock? (Warming it maybe? To what temperature?)

[bob1]

I just weigh out the required amount onto a piece of sterile tinfoil/aluminium foil and drop this into the solution, then remove when the triton has dissolved.

[steveneam]

Try cutting the pipette tip off with scissors (around a third of the way) to allow for a larger opening. That should also allow you to draw up th soln much easier.

[pathlily]

bob1, on 17 August 2011 - 03:09 PM, said:

I just weigh out the required amount onto a piece of sterile tinfoil/aluminium foil and drop this into the solution, then remove when the triton has dissolved.

so.. (to be pedantic about this): tinfoil from the roll is assumed to be sterile, right?

Once you've dispensed the required weight of liquid onto the tinfoil and dropped it into the solution, do you just wait for it to dissolve at RT on the bench or do you warm and swirl it or stir it with a flea?
Then, if I get you correctly, you remove the foil and make up to the required volume?

[pathlily]

steveneam, on 17 August 2011 - 03:39 PM, said:

Try cutting the pipette tip off with scissors (around a third of the way) to allow for a larger opening. That should also allow you to draw up th soln much easier.

that's a good idea, thanks, I'll give it a try.

[bob1]

pathlily, on 17 August 2011 - 03:49 PM, said:

bob1, on 17 August 2011 - 03:09 PM, said:

I just weigh out the required amount onto a piece of sterile tinfoil/aluminium foil and drop this into the solution, then remove when the triton has dissolved.

so.. (to be pedantic about this): tinfoil from the roll is assumed to be sterile, right?

Once you've dispensed the required weight of liquid onto the tinfoil and dropped it into the solution, do you just wait for it to dissolve at RT on the bench or do you warm and swirl it or stir it with a flea?
Then, if I get you correctly, you remove the foil and make up to the required volume?

I would autoclave bits of tinfoil in a small bag or container if you need it to be sterile.  I swirl to dissolve, remove foil with sterile forceps and make up to the required volume.

[pathlily]

Thanks!