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Butyl-agarose/sepharose purification

protein purification chromatography

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#1 Starbrow

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Posted 17 August 2011 - 10:22 AM

Has anyone ever used a butyl-agarose or butyl-sepharose column as an intermediate or polishing step in protein purification?

I'm looking for protocols and general pointers.

Thanks!

#2 mdfenko

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Posted 18 August 2011 - 11:08 AM

you can look it up in this handbook (free download) from ge healthcare:

hydrophobic interaction and reversed phase handbook

Edited by mdfenko, 18 August 2011 - 11:11 AM.

talent does what it can
genius does what it must
i do what i get paid to do





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