how do i make more competence cell from my E.coli BL21(DE3) competent cell? by growong up and glycerol stock? for plasmid transformation and protein expression proposal...
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Legendary Graduate Beggar
Posted 15 August 2011 - 07:31 PM
As usual, grow it, and use Calcium chloride method or others as shown:
http://openwetware.o...competent_cells
http://openwetware.o...lls_%28Inoue%29
http://openwetware.o...competent_cells
http://openwetware.o...lls_%28Inoue%29
Hope this helps.
http://openwetware.o...competent_cells
http://openwetware.o...lls_%28Inoue%29
http://openwetware.o...competent_cells
http://openwetware.o...lls_%28Inoue%29
Hope this helps.
Expecting the world to treat you fairly because you are a good person is like expecting the lion not to attack you because you are a vegetarian.
..."best of our knowledge, as far as we know this had never been reported before, though I can't possible read all the published journals on earth, but by perform thorough search in google, the keywords did not match any documents"...
"what doesn’t kill you, makes you stronger"---Goddess Casandra reminds me to be strong
"It's all just DNA. Do it."---phage434
..."best of our knowledge, as far as we know this had never been reported before, though I can't possible read all the published journals on earth, but by perform thorough search in google, the keywords did not match any documents"...
"what doesn’t kill you, makes you stronger"---Goddess Casandra reminds me to be strong
"It's all just DNA. Do it."---phage434
