Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

competentce cell, cloning

Competence cell growing

  • Please log in to reply
2 replies to this topic

#1 Yilmaz Kaya

Yilmaz Kaya

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 15 August 2011 - 05:24 PM

how do i make more competence cell from my E.coli BL21(DE3) competent cell? by growong up and glycerol stock? for plasmid transformation and protein expression proposal...

#2 Adrian K

Adrian K

    Legendary Graduate Beggar

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 707 posts
28
Excellent

Posted 15 August 2011 - 07:31 PM

As usual, grow it, and use Calcium chloride method or others as shown:

http://openwetware.o...competent_cells
http://openwetware.o...t_cells_(Inoue)

http://openwetware.o...competent_cells
http://openwetware.o...t_cells_(Inoue)

Hope this helps.
Expecting the world to treat you fairly because you are a good person is like expecting the lion not to attack you because you are a vegetarian.

..."best of our knowledge, as far as we know this had never been reported before, though I can't possible read all the published journals on earth, but by perform thorough search in google, the keywords did not match any documents"...

"what doesn't kill you, makes you stronger"---Goddess Casandra reminds me to be strong

"It's all just DNA. Do it."---phage434

#3 Yilmaz Kaya

Yilmaz Kaya

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 16 August 2011 - 07:42 PM

Thanks Adrian...




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.