I recently PCRed my gene of interest (the complete form of the gene) from genomic DNA and now another step is to only amplify the gene encoding for the mature form of my gene and then ligate into an expression vector...how do I ONLY amplify the mature form..wouldn't I essentially do the exact same thing as the first time I did PCR the only diff. being I'll be ligating into an expression vector? And then the mature protein will be produced once in expression vector?? Hope I'm making sense...
PCRing mature form of a gene
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