Transient Transformation of Nicotiana benthamiana leaves
Posted 15 August 2011 - 10:35 AM
Everything I found out was that if you infiltrate the leaves with Agrobacteria that carry a plasmid with your desired gene, the gene sequence is not intergrated into the chromosome... But where does the gene expression takes place then? I the agrobacteria which are in the plant cells?
So consider you want to express a gene that carries introns. In bacteria is no splicing mechanism and so your gene expression wouldn't work with this technique.
I hope someone can help me with my problem!
Posted 14 September 2011 - 08:00 PM
Edited by kajmak, 14 September 2011 - 08:01 PM.