I need this kind of professional help.
Recently i've noticed a protocoll to reuse the Transwell inserts, and a pretty basic one (can't find an appropriate link - but it suggested by J. Celis, 2006): 1. put the inserts in the detergent TritonX for 24 h; 2. wash and hold in the distilled water for 1h; 3. put the insert in the 70% ethanol overnight under the UV-light in the bench.
since i could find only SDS as a detergent, tried it out - and it worked fine to me, but the last time i saw loads of particles, probably the remains of cell, and numerous cells. Well, the cells were fixed with paraformaldehyde. now i'm trying out 2% SDS instead of 1% solution.
Do you have by chance an another protocol to reuse the transwell inserts in mind? Can u suggest me maybe an another, stronger detergent (rather than SDS) found in every lab i could experiment with?
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