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Co-immunoprecipitation


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#1 paulis

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Posted 14 August 2011 - 07:49 PM

Hi there,

I'll be doing a co-inmunoprecipitation, and I would like to know 2 things:
- what concentration of bead is used in relation to amount of protein? And
- what amount of antibody is used for the concentration of bead used?

thanks!

#2 Curtis

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Posted 14 August 2011 - 11:16 PM

20 ul of beads with 1-1.5 ug of antibody




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