PCR products form with DNA, but not with c-DNA
Posted 12 August 2011 - 02:15 PM
I am currently trying to test gene expression of a transgene (inserted in poplar) using q-PCR. I designed primers and tested them first on DNA and obtained bands on an agarose gel (got bands for the positive control as well). Then, I tried testing the primers on c-DNA. While I got bands for ubiquitin, I did not get any bands for my primers. What could be the possible reasons? Any advice on how to proceed would be appreciated. I have the gel pictures and can upload them if required.
Thanks in advance.
Posted 14 August 2011 - 04:27 AM
Other reason may be, that your transgene is simply not expressed.
I never trust anything that can't be doubted.
Posted 16 August 2011 - 02:59 PM