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insert loss


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#1 molimm

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Posted 29 December 2002 - 07:03 AM

Hi All,

I have cloned some high GC content DNA (64% approx) from Leishmania parasite in pUC18 and transformed in XL1-Blue. The insert is getting lost during next round broth culture or plating. Can any body suggest some remedy?

Thanks

#2 lzfly

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Posted 29 September 2003 - 05:39 AM

I also clone GC rich DNA (70%) and met the same problem, may be the unclonable DNA have secondary structure.I want to try another host strains such as SURE (stratagene) or STBL2 (GIbico)




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