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hyperchromicity assays for DNAse activity


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#1 Gaai

Gaai

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Posted 10 August 2011 - 07:05 AM

Dear all,

I'm trying to characterize a (very) low rate nuclease using hyperchromicity and it gives me headaches :( . The definition of a (kunitz) Unit using this methodoloy is not very clear, as depending on the source, people are using different substrate concentrations and assay conditions. I generally do my assays using 50g/ml gDNA in the optimal pH and cofactor conditions for my enzyme. My question is whether I should calculate the activity by multiplying the mAU260nm/minxmg by 20 to extrapolate to 1 mg/ml gDNA substrate?
Thank you so much for your suggestions




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