Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

type of IgG in my sample??


  • Please log in to reply
1 reply to this topic

#1 rick112

rick112

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 71 posts
0
Neutral

Posted 09 August 2011 - 03:56 AM

hi...

i have protein A purified samples of antibody raised against my protein,any suggestion on a simple method by which i could find about types of IgG i have in my samples??

also could any one suggest me methods to purify antibodies specific to my protein??

thanks a lot

#2 BioMiha

BioMiha

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 236 posts
9
Neutral

Posted 09 August 2011 - 05:28 AM

What do you mean by "types of IgG i have in my samples??"? Are you interested in the IgG subclasses. In that case, you can do ELISA with subclass-specific secondary antibodies.
Concerning the purification of specific Abs, if you have enough protein (the antigen used to make the Abs), then you can immobilize the antigen to activated agarose beads and make an affinity column.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.