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How many RNA I need for detecting plasma miR expression?

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#1 txyuan



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Posted 08 August 2011 - 02:43 PM

Hi, i am working on miR expression in plasma. I used miRNesay to extract miRNA, but i only got 30-100ng RNA per 200ul plasma(mice). I am planning to use microarray to test miR expression. But the core facility asked me to provide 5ug RNA at least for each chip. That is too much. Because I usually get 500-600ul plasma from each mouse, and the max amount of RNA is 300ng/mouse. So if I want to get 5ug, I must sac 20 mice for one chip.
Have anyone done the similar research? How many RNA usually you put on one chip?
The concentration of RNA from plasma is around 5-10ng/ul, could I condense RNA? How?

#2 David C H

David C H


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Posted 10 August 2011 - 05:54 AM

Use a kit to amplify your RNA. The MessageAmp II kit from Ambion/Applied Biosystems is commonly used for this:


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