Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo

How many RNA I need for detecting plasma miR expression?


  • Please log in to reply
1 reply to this topic

#1 txyuan

txyuan

    member

  • Members
  • Pip
  • 3 posts
0
Neutral

Posted 08 August 2011 - 02:43 PM

Hi, i am working on miR expression in plasma. I used miRNesay to extract miRNA, but i only got 30-100ng RNA per 200ul plasma(mice). I am planning to use microarray to test miR expression. But the core facility asked me to provide 5ug RNA at least for each chip. That is too much. Because I usually get 500-600ul plasma from each mouse, and the max amount of RNA is 300ng/mouse. So if I want to get 5ug, I must sac 20 mice for one chip.
Have anyone done the similar research? How many RNA usually you put on one chip?
The concentration of RNA from plasma is around 5-10ng/ul, could I condense RNA? How?
Thanks.

#2 David C H

David C H

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 54 posts
1
Neutral

Posted 10 August 2011 - 05:54 AM

Use a kit to amplify your RNA. The MessageAmp II kit from Ambion/Applied Biosystems is commonly used for this:

http://www.ambion.co...CatNum.php?1751




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.