Gene Expression Issues
Posted 08 August 2011 - 07:43 AM
Posted 10 August 2011 - 12:04 PM
Posted 10 August 2011 - 02:32 PM
But doesn't rRNA make up >80% of the RNA in cells? Since 18s is a rRNA, I thought that it would have a very high expression. Could the Alu sequences have higher expression than the rRNA? Does the 293t cell line contain less rRNA than other cells?
Posted 11 August 2011 - 01:25 AM
Other thing may be, if your Cts are really low (< 15), that 18S is so abundant it inhibits the reaction. If you dilute your sample like 500 - 1000x you may get Ct values in the safer range.
I never trust anything that can't be doubted.