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Cleaved caspase 3 and AnnexinV


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#1 tali

tali

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Posted 06 August 2011 - 10:06 PM

Hi,
I am trying to detect Apoptosis by Facs (AnnexinnV) and by western blot (Cleaved caspase 3) and want to cross their appearance. However, I could not find in the literature the time line. I know AnnexinV appears at the early stage of apoptosis. Caspase3 cleavage is also in the early± stages. Isn't it?
So, if I want to find both markers, what comes first?
TNX
Tali




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