I would like to measure the testosterone level in TM3 (mouse leydig cell) culture medium using ELISA while this metabolic-active cell can turn the medium yellow in one day. I think the medium should be adjusted back to ~pH 7.4 for the ELISA but I don't know the best way to do so.
I think the simplest way is to neutralize by NaOH, but in some of the assays, I use medium without phenol red, so I have to check the pH of my samples using pH paper one by one. Can I neutralize using buffer in high concentration like Tris-HCl?
For example, add 10 ul 1M Tris-HCl (pH 7.4) to 1 ml medium and assume all samples being adjusted to ~pH 7.4.
Thank you very much!
Edited by kkmans, 06 August 2011 - 06:04 AM.