Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Combination of secondary antibodies


  • Please log in to reply
2 replies to this topic

#1 mik-i

mik-i

    member

  • Active Members
  • Pip
  • 9 posts
0
Neutral

Posted 05 August 2011 - 06:23 AM

Ciao!

I just read something and I think authors made a mistake, but I'm not sure: can you combine a FITC-labeled Goat anti-Rabbit Ab with a TRITC-labeled Rabbit anti-Mouse Ab? Won't they react with each other, giving false positives as a result? So, one might think he has co-localizes two proteins, but actually it's just the two secondary antibodies reacting? Or is the fluorescent marker preventing binding?

Greetz,
Kim

#2 mdfenko

mdfenko

    an elder

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 2,811 posts
136
Excellent

Posted 05 August 2011 - 06:43 AM

you're correct. the anti-rabbit should interact with the anti-mouse. maybe the authors stripped between probes?
talent does what it can
genius does what it must
i do what i get paid to do

#3 mik-i

mik-i

    member

  • Active Members
  • Pip
  • 9 posts
0
Neutral

Posted 05 August 2011 - 07:10 AM

you're correct. the anti-rabbit should interact with the anti-mouse. maybe the authors stripped between probes?



Ciao!

Thanks! I thought so already, but wanted to check! If they did the stripping, then they certainly didn't mention anything about it? Strange.

Bye!
Kim

P.S.: love your quote!




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.