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SVZ culture


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#1 napa161

napa161

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Posted 04 August 2011 - 10:18 PM

I have some problem when i try to culture SVZ stem cell.
after i dissect SVZ, trypsinize and add medium, i observe that
medium culture became pink and turbid. next day, the white mucus appear.
I have this problem everytime. I think it might be comtamination but i cannnot observe
the foreign body under microscope.
after i passage this group of cell, cells decrease growth rate and death.
- my medium contain pen/strep, i might be the cause the i cannot observe the contamination?
Can anyone help me to cure this problem? Please!!!




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