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Fluorescence at 560


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3 replies to this topic

#1 jerseysurfer66

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Posted 04 August 2011 - 01:23 PM

This is the fluorescence profile Iím getting with my protein bound vs. unbound. Any ideas on what that bump at 560 is?

#2 bob1

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Excellent

Posted 04 August 2011 - 05:08 PM

You forgot to attach the profile...

Can you also provide a baseline profile (i.e. no interaction)

#3 jerseysurfer66

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Posted 04 August 2011 - 06:13 PM

The Lower curve is protein alone. The ligand does not fluoresce. It's not ultra important what it is as my quench flow kinetics make sense and agree with my NMR data. I'm just wondering what that little blip at 560 might be.

#4 mdfenko

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Excellent

Posted 05 August 2011 - 06:32 AM

the profile is still not attached. we hard to analyze what we can't see.

is 560 the excitation or emission wavelength?

is the blip before or after the peak wavelength?

there could be some quenching or conformational change or ...

Edited by mdfenko, 05 August 2011 - 06:33 AM.

talent does what it can
genius does what it must
i do what i get paid to do




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