Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

LPS and Ig class switching


  • Please log in to reply
No replies to this topic

#1 ginkgo leaf

ginkgo leaf

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 04 August 2011 - 11:35 AM

Hello, I've been stuck on this for some time, and can't find a definitive answer. I am culturing mouse splenocytes to assay antibody production after stimulation with LPS alone or in combination with cytokines. I understand that LPS+cytokines induces isotype switching to specific isotypes, but what does LPS do alone? I've read many times that it both causes polyclonal B cell proliferation (in high doses) and induces class switching, but does it cause switching to specific isotypes? The data I currently have is not very reliable, and papers I've seen only consider LPS in combination with other factors. Do any of you have any experience/knowledge in this area? I would appreciate any help/advice you could provide, thank you!




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.