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Housekeeper for Plasma samples in Western Blotting

Started by MikeP, Aug 03 2011 01:36 PM

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#1 MikeP

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Posted 03 August 2011 - 01:36 PM

Hi Everyone

I'm currently trying to run a Western Blot on some Asthma and COPD serum samples. Since beta actin and GAPDH are secreted protiens and can't be used as the houskeeper, does anyone have any idea what could be used.

Any help would be greatly appreciated

Thanks

#2 blackstone

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Posted 03 August 2011 - 10:56 PM

how about the albumin?

#3 MikeP

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Posted 04 August 2011 - 12:24 AM

View Postblackstone, on 03 August 2011 - 10:56 PM, said:

how about the albumin?

Thanks - that was one I was considering

#4 mdfenko

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Posted 04 August 2011 - 06:33 AM

albumin concentration may not be consistent enough between samples to use for normalization
talent does what it can
genius does what it must
i do what i get paid to do
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