Recently we have been experiencing problems with protein transfer using our Invitrogen Surelock system. The right hand side (last four lanes) of the gel are not transferring to the membrane correctly, it is coming out very weak in comparison to the left hand side. If we run the ladder on the right a lot of the dye will remain on the gel, yet will transfer cleanly when loaded on the left. This problem is consistent with all members of the lab. I thought that maybe there could be oxidation on one or both of the metal plates in the transfer box that is impeding current flow at that particular spot, but cannot see any oxidation at all. I have called Invitrogen, but they claim to have not seen this problem before. Has this happened to anyone else, does anyone have any suggestions?
I don't think it is the sandwich construction or sandwich pads as it is always on the right hand side, we always use the same transfer buffer recipe, running times and voltages.
Any help much appreciated.
Edited by stelane, 02 August 2011 - 01:48 PM.