2 replies to this topic

[Biogareth]

Hello all,

Recently I read some information about succinate dehydrogenase, which reversiably converts succinate into furmate. This kind of enzyme always contain four subunits: A subunit is succinate binding site attached with FAD; B subunit contains three iron-sulfur clusters; Subunit C and D are hydrophobic membrane anchor subunits, containing one heme b group and a ubiquinone-binding site.
I don't know is it possible to recombinant express only subunitA for activity test?

Every suggestion is welcome!

Thanks in advance!

Edited by Biogareth, 02 August 2011 - 07:19 AM.

[Biogareth]

Waiting discussions!

[mdfenko]

it may. it depends on how the other subunits regulate the active subunit.