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Primer design


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3 replies to this topic

#1 MurphysunHKU

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Posted 29 July 2011 - 09:08 PM

Hi everyone,

Is there any software or program that is good for primer design and can allow me to see whether there is hairpin formed or complementary sequences between two primers.

#2 phage434

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Excellent

Posted 30 July 2011 - 06:04 AM

The "Tools" menu on the IDT web site has a nice tool for this. http://idtdna.com

#3 pcrman

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Excellent

Posted 30 July 2011 - 10:18 PM

Here you are: Biotool on this site - http://www.protocol-...imer_stats.html

#4 Evanescence

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Posted 07 August 2011 - 02:19 AM

try to google "oligonucleotide calculator" or just paste this "http://www.basic.nor.../oligocalc.html " into your box...
it gives u a tool to calculate Tm, GC content, blast primer, complementary or primer dimer possibilities...etc..just check it very useful!!

Have a good try

Evanescence
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