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Dissolve DNA from DGGE bands


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#1 sxh999

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Posted 29 July 2011 - 05:32 PM

Hello everyone,

I run DGGE using my PCR products (from genomic DNA), I cut bands in the DGGE gel, after that, I need to dissolve the DNA in the bands and repeat PCR, then sequencing.

Please give me suggestions what should I use to dissolve DNA in the DGGE gel, at the same time there should be no reverse impact on the sequent PCR and sequencing.
Thank you so much.

Good luck to everyone.

Holly





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