Hi
we have HCV genes expression vectors and we have screened siRNA activity against it. Now I want to make their luciferase DNA constructs to screen against shRNAs. Can any body tell me how should I proceed|?
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Help to construct luciferase DNA construct
Started by sultan, Jul 28 2011 09:18 PM
2 replies to this topic
#2
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Epigenetist
Posted 30 July 2011 - 09:54 PM
Since you are doing RNAi using siRNA or shRNA, you can simply measure your target gene expression and don't need to put the target into a reporter (3UTR).
#3
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Posted 08 August 2011 - 08:45 PM
pcrman, on 30 July 2011 - 09:54 PM, said:
Since you are doing RNAi using siRNA or shRNA, you can simply measure your target gene expression and don't need to put the target into a reporter (3UTR).
Thanks pcrman for your kind reply. You are right but when we see literature regarding shRNA silencing, in almost most of the literature people have mostly measured the inhibitory effect of shRNA by making DNA constructs in luciferase reporters while I have the cloned the structural genes of HCV in PCR 3.1. Can you kindly suggest how i should proceed?
