problems on the media for oryza sativa(rice)
Posted 28 July 2011 - 06:34 PM
i wanna ask a Q on the problems happened to my CIM (callus induction media)
after about a 2-3weeks i cultured the seeds of Oryza sativa, i already got the calli and i subcultured the calli to new media.
but then, after about 1week, the calli turned yellow/brown color as well as the media.
why actually this happen? i'm worried already
is it the technique used or the problem with the media??
anyone,please help.. tq
Posted 01 August 2011 - 08:34 AM
Posted 16 August 2011 - 08:38 AM
another common problem was crystallisation of the tissue, kind of what happens to the politician in the x-men movie...