Direct sequencing PCR product
Posted 27 July 2011 - 02:18 PM
My boss wants us to save money instead of using expensive TOPO kits. I was wondering if anyone has sequenced PCR products from bisulfite treated DNA?
Otherwise, what cloning vectors do you use for bisulfite sequencing?
Posted 30 July 2011 - 10:15 PM
Posted 31 July 2011 - 05:44 PM
All comments and communication are my own personal ones, and are not tied to any of my affiliations.
Posted 01 August 2011 - 07:21 AM
I would prefer to sequence from clones but cost is now a big issue. Will see how much pGEM is