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cDNA synthesis + amplification


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#1 amandasmith05

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Posted 27 July 2011 - 12:40 PM

I'm trying to synthesize cDNA from a small amount of RNA so I would like to amplify it as well... What is the best kit/way to do this?

Also, do people usually clean up their cDNA products? Why or why not? I'm losing a lot of yield with the Qiagen column.

Thanks!

#2 pcrman

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Posted 30 July 2011 - 10:28 PM

If you start with small amount RNA, I would suggest that you do two step RT-PCR--first do RT to convert RNA into cDNA, and then amplify your target genes using the cDNA as template by PCR. You don't need to purify your cDNA after the RT reaction.

#3 amandasmith05

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Posted 02 August 2011 - 07:35 AM

Ah, thanks!




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