2 replies to this topic

[amandasmith05]

I'm trying to synthesize cDNA from a small amount of RNA so I would like to amplify it as well... What is the best kit/way to do this?

Also, do people usually clean up their cDNA products? Why or why not? I'm losing a lot of yield with the Qiagen column.

Thanks!

[pcrman]

If you start with small amount RNA, I would suggest that you do two step RT-PCR--first do RT to convert RNA into cDNA, and then amplify your target genes using the cDNA as template by PCR. You don't need to purify your cDNA after the RT reaction.

[amandasmith05]

Ah, thanks!