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Viral transfection to neurosphere in vitro


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#1 Rnotk

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Posted 27 July 2011 - 10:07 AM

One of my friends asked me about viral transfection to primary neurosphere in vitro, but I dont have experience to that.

Does anyone ever did similar experiment? Anything that she needs to be careful about like selection of virus and such?
or any paper to read before design virus?

Any suggestion helps

Thanks in advance

#2 CloneMan

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Posted 15 September 2011 - 06:01 PM

This 2002 Molecular Therapy article compares 3 different virus for infecting primary neurosphere in vitro - feline immunodeficiency virus (FIV), adeno-associated virus (AAV) or adenovirus (Ad) and it seems adenovirus works the best.

http://www.nature.co...df/mt20025a.pdf

But the conclusion could be different for in vivo infection.




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