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What transcription factors are involved in transient transfection


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#1 Gangwolf

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Posted 27 July 2011 - 08:44 AM

Hi All!
To be more specific; I am using a vector containing the CMV expression promoter and transient transfect that into HEK293 cells.

#2 Evanescence

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Posted 04 August 2011 - 08:53 PM

Maybe you can try this website link

http://linux1.softbe...com/berry.phtml @ www.softberry.com
it is a good site to predict prokayote, eukaryote promoters, protein structure,even in 3D and so many free application there..
you should try maybe it provides you an answer

by the way you also can use this website to predict the promoter and transcription factor
www.fruitfly.org/seq_tools/promoter.html

you must choose for use in eukaryote or prokaryotes system...the site will give you a few predicted prmoters and transcriptional factors
within Neural network promoter sequence (shown in the result)

I used both sites (softberry and fruitfly) to reconfirm...

try one and maybe the solution is there:)
good luck

Fortune is easy to come if we find it
or luck we may get by chance but it rare

Evanecence




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