Freezing culture media
Posted 27 July 2011 - 04:04 AM
When I open a new 500ml bottle (doesn't come in smaller sizes) I add L-Glutamine from a frozen aliquot, and when I use it I tend to make 50ml aliquotes of 20% FCS in MCDB (i.e. 10ml FCS and 40ml MCDB). I make 1 50ml aliquot at a time, so when I finish using the 50ml I decant out a new 40ml MCDB and 10ml FCS.
The problem I am having is that I don't use it especially fast, perhaps a 500ml bottle every 40-50 days, and use it gradually and slowly within that period. However by the time I get to the last 1/3 or so of the bottle the media has started to go a pinkish colour indicating it is going off, and the cells don't grow anywhere near as well as they do when the media is fresh. When I open a new bottle I add L-Glutamine and I assume this is also going off within that time.
I am wondering if it would be a good idea to make my 50ml aliquots at the start of opening the bottle then freeze them down, to preserve the L-Glut and other componants of the media. I hve not heard of anyone freezing media before but just wondering if it is possible or if it would damage various media componants.
Posted 27 July 2011 - 04:45 AM
Posted 27 July 2011 - 05:24 AM
Posted 27 July 2011 - 06:25 AM
Posted 20 September 2011 - 08:37 AM