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DNase Treatment of RNA isolated fron LCM samples


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#1 genie

genie

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Posted 27 July 2011 - 12:38 AM

Hi All,
After great difficulty I was finally able to isolate RNA from Laser Capture micro-dissected samples using Trizol method, (trials with Micro kits were unsuccessful). my Total RNA yield measured using Qubit fluorometer was around 80ng in 10ul. Im planning to do q-pcr using Taqman probes (_m ending primers), in this case will I still have to a DNase treatment? I fear this could result in greater loss of the RNA.
Can anybody give me some guiding advice?
Thanks




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