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chip precipitation


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#1 ford44

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Posted 26 July 2011 - 09:04 AM

At the end of PK step on chip experiment and as I was using 1.5 ml eppendorf and my volume of eluted was 550 ul I used isopropanol 0.8 vol with 1/10 AcONa 3M and glycogen, I put in the freeze -80, centrifuge but I could not see any pellet. I let the tubes at -20C o/n. question: It was a bad idea to use isopropanol, I need more alcohol volume to precipitate the DNA, Waht could I do to repare the missfit?.
Thank you.

#2 chabraha

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Posted 28 July 2011 - 08:11 AM

sounds to me like you may have skipped a step........like cleaning up the PK reaction with a phenol:chloroform:isoamyl extraction, then go on to precipitate your DNA with EtOH. Or even simpler, after your PK reaction, use a Qiagen column.
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