Edited by blossoms123, 26 July 2011 - 05:09 AM.
mouse bone marrow MSC culture
Posted 26 July 2011 - 05:08 AM
Posted 16 August 2011 - 03:34 PM
But in your case, cell is dying, so first of all, I will make sure the initial seeding density, then will check the medium (especially quality of FBS)
Posted 23 September 2011 - 01:29 AM
I flush bone marrow of 1 femur bone and seed in one 100mm dish.
please suggest what changes i need to make for cells to grow?
Posted 29 September 2011 - 10:56 AM
I think the seeding density might be too low.
Do you normally count viable cells before seeding?
Medium sounds fine to me, but also have you ever seen any contamination?
Posted 20 May 2012 - 07:02 AM
But, i face a new problem.
m unable to detach all cells from a T25 flask by Trypsin EDTA. I keep the trypsin at 37degree b4 using. I wash the cells twice with TPVG and add 2ml more TPVG and keep for 2-3 min at 37 degree but still all cells don't detach.
Due to repeated TPVG exposure cells are also getting stressed.
Please pls.. help.
Posted 20 May 2012 - 08:09 AM
Posted 17 September 2012 - 10:28 PM