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Spec problems


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#1 vwracer06

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Posted 04 December 2002 - 12:32 PM

When i go to measure protein concentration, i keep getting negative 260 and 280 numbers. Then i decided to just try using water as a blank and then a titration of just BSA and i still keep getting negative numbers. Anyone know why or what it means? how can i have a negative abs?

#2 madhujit

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Posted 07 December 2002 - 10:12 PM

are u using urea in any of your steps cos i have encountered such a problem with 6M urea u can also take a fluoresence scan of yor protein some materails that may interfere with 280 abs do not do so with fluoresence.

#3 lifengzhang

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Posted 05 March 2003 - 03:45 PM

You can use a new measure kit from Biorad, it is special for 2-D running, it is compactble to urea.




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