While preparing freezing media(DMSO +FCS)and due to mishandling a small amount of trypsin is mixed in the freezing media.
Will this trypsin in freezing media show any effect to the cells during long time storage.
Thanx in advance
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#2
Gangwolf
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Posted 04 January 2012 - 07:36 AM
No! Trypsin should not have any enzymatic activity at freezing temperatures. However, wash away the trypsin prior to seeding your frozen cells.
#3
bob1
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Posted 04 January 2012 - 12:40 PM
The trypsin should also be neutralized by the addition of the FCS.
