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freezing adherant cells


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#1 harry348

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Posted 25 July 2011 - 01:47 AM

While preparing freezing media(DMSO +FCS)and due to mishandling a small amount of trypsin is mixed in the freezing media.
Will this trypsin in freezing media show any effect to the cells during long time storage.

Thanx in advance

#2 Gangwolf

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Posted 04 January 2012 - 07:36 AM

No! Trypsin should not have any enzymatic activity at freezing temperatures. However, wash away the trypsin prior to seeding your frozen cells.

#3 bob1

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Posted 04 January 2012 - 12:40 PM

The trypsin should also be neutralized by the addition of the FCS.




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