What exactly does BSA do in immunohistochemistry?
Posted 24 July 2011 - 06:15 PM
Posted 25 July 2011 - 09:59 PM
Posted 27 July 2011 - 07:09 AM
Blocking with BSA first means that all these non-specific sticky proteins are saturated with the high concentration of BSA that you have added, leaving your antibody free to bind specifically to your protein of interest. BSA is used mostly because it is cheap and abundent, people often also use plain old milk powder from the local cornershop as it contains a mixture of random nonspecific proteins found in milk, good for blocking in ELISAs and such. (Although I guess you wouldn't use opaque milk powder for highly sensitive immunohistochemistry)