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How the heck does serum activate Akt?!?


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#1 alwayslearning

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Posted 24 July 2011 - 05:48 AM

I've been searching and searching for literature on reasons why I didn't see Akt activation in response to VEGF stimulation in HUVECs. The only thing I can come up with is that I didn't serum starve the cells prior to stimulation. Growth factors in the serum activate pathways that are Akt dependent? That's my guess but I can't find anything to back it up. Any insight?
Thanks,
Katie

#2 Inmost sun

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Posted 26 July 2011 - 03:33 AM

how do you run controls? how do you check activation of Akt?




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